Isolation and characterization of PSII core complexes from a brown alga, Laminaria saccharina

FEBS Letters

Berkaloff, Claire¹  Douady, Dominique¹  Rousseau, Bernard¹ 

[1] Laboratoire des biomembranes végétales, U A CNRS 311, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France

关键词: Brown alga;    Chromophyte;    D1;    PSII;    Pigment-protein complex;    bistris;    2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-propane-1.3-diol;    Chl;    chlorophyll;    CP43;    CP47;    D1;    D2;    polypeptides components of green plant PSII complexes;    DCIP;    2;    6-dichlorophenolindophenol;    DEAE;    diethylaminoethylcellulose;    DPC;    2.2'diphenylcarbazide;    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethanesulphonic acid;    LiDS;    lithium dodecyl sulphate;    PS;    photosystem;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(93)81524-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PSII-enriched particles, active for DCIP-reduction, were prepared from Laminaria saccharina chloroplasts, and PSII core complexes were further purified by ion-exchange chromatography. They contained several polypeptides, four of them cross-reacting with antibodies raised against CP47, CP43, D1 and D2 of green plants. A second chromatography was required to separate: (i) a core antenna, composed of 51 kDa polypeptide subunits, binding 11 β-carotene, 4 chlorophyll (Chl) c and 7 fucoxanthin for 100 Chl a, and reacting with CP47 antibodies; and (ii) a reaction center complex consisting of two main polypeptides of 34 and 36 kDa. The pigment stoichiometry was of 5 Chl a and 0.5 β-carotene for 2 pheophytin a. The 34 and 36 kDa components cross-reacted with anti-D1 and anti-D2 antibodies, respectively. The presence of cytochrome b-559 was substantiated by spectrophotometry.

【 授权许可】

Unknown