Modification of the chloride requirement for photosynthetic O2 evolution

FEBS Letters

Critchley, Christa²  Larsson, Christer³  Anderson, Jan M.⁴  Andersson, Bertil⁴  Jansson, Christer³  Ryrie, Ivan J.¹ 

[1] Department of Environmental Biology, Australian National University, Canberra, ACT 2601, Australia;Botany Department, Australian National University, Canberra, ACT 2601, Australia;Department of Biochemistry, University of Lund, S-22007 Lund, Sweden;CSIRO Division of Plant Industry, Canberra, ACT 2601, Australia

关键词: Photosynthetic oxygen evolution;    Chloride requirement;    23 kDa protein;    Western blotting;    Inverted thylakoid vesicle;    Phase partitioning;    AMPD;    2-amino-2-methyl-1;    3-propanediol;    LiDS-PAGE;    lithium dodecyl sulphate-polyacrylamide gel electrophoresis;    FeCN;    ferricyanide;    PBQ;    phenyl-p-benzoquinone;    Chl;    chlorophyll;   

DOI  :  10.1016/0014-5793(84)80217-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In thylakoid preparations from spinach and the halophyte Avicennia marina a correlation is observed between functional O₂ evolution at low chloride concentrations and the presence of the 23 kDa protein. Addition of spinach 23 kDa protein to polypeptide-depleted halophyte inside-out thylakoid vesicles lowers their chloride requirement for optimal O₂ evolution activity from 250 to 5 mM. It is suggested that the specific role for the 23 kDa protein is to increase the affinity of the water oxidation site for chloride.

【 授权许可】

Unknown