Evidence for the presence of a component of the Mn complex of the photosystem II reaction centre which is exposed to water in the S2 state of the water oxidation complex

FEBS Letters

Rich, Anne M.¹  Evans, Michael C.W.¹  Nugent, Jonathan H.A.¹ 

[1] Department of Biology, University College London, Gower St., London WC1E 6BT, UK

关键词: Photosystem II;    Water oxidation;    Manganese complex;    Photosynthesis;    Photosynthetic oxygen evolution;    Electron spin echo envelope modulation;    EPR;    electron paramagnetic resonance;    ESEEM;    electron spin echo envelope modulation;    XAS;    X-ray absorption spectroscopy;   

DOI  :  10.1016/S0014-5793(00)01742-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of water oxidising photosystem II preparations with the aqueous environment has been investigated using electron spin echo envelope modulation spectroscopy in the presence of ²H₂O. The spectra show interaction of ²H of ²H₂O with the preparation in the S₂ state. The component interacting with water decays during 1–4 weeks storage at 77 K. No interaction of water with the classical multiline S₂ Mn signal, which is more stable on storage at 77 K, was detected. The results show that a component of the water oxidation complex, possibly involving the Mn centre, is accessible to water and may be the water binding site for photosynthetic water oxidation.

【 授权许可】

Unknown