The release of a 10‐kDa polypeptide from everted photosystem II thylakoid membranes by alkaline tris

FEBS Letters

Åkerlund, Hans-Erik¹  Andersson, Bertil¹  Ljungberg, Ulf¹ 

[1] Department of Biochemistry, University of Lund, PO Box 124, S-221 00 Lund, Sweden

关键词: Thylakoid polypeptide;    Water oxidation;    Photosystem II;    10-kDa polypeptide;    Tris washing;    Oxygen-evolving complex;    MES;    2-(N-morpholino)ethanesulphonic acid;    PMSF;    phenylmethylsulfonylfluoride;   

DOI  :  10.1016/0014-5793(84)80746-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The release of polypeptides from inside-out thylakoid vesicles and photosystem II by alkaline Tris treatment was reinvestigated, using SDS-polyacrylanude gel electrophoresis (PAGE) in the presence of urea, with highly increased resolution in the low molecular mass region. In addition to the 33-, 23-, and 16-kDa proteins of the oxygen-evolving complex, a 10-kDa polypeptide was released. This 10-kDa polypeptide is an entrinsic polypeptide located at the inner grana thylakoid surface and with a likely role in photosynthetic oxygen evolution.

【 授权许可】

Unknown