Effectivity of immunostimulant from Zoothamnium penaei protein membrane for decreasing the mortality rate of white shrimp (Litopenaeus vannamei) in traditional plus pond

Asean-Fen International Fisheries Symposium - 2017

Mahasri, G.^1 ; Kusdarwati, R.^1 ; Kismiyati^1 ; Rozi^1 ; Gustrifandi, H.^2

Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Campus C, Mulyorejo Surabaya, Indonesia^1

Fish Quarantine Center and Quality Control of Fishery Products, Class i, Surabaya, Indonesia^2

关键词: Experimental laboratory;    Immune response;    Litopenaeus vannamei;    Membrane proteins;    Mortality rate;    Protein membrane;    Random sampling;    Western blotting;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012020/pdf
DOI  :  10.1088/1755-1315/137/1/012020

来源: IOP

【 摘 要 】

The purpose of this research was to analys immunogenic membrane protein as immunostimulant development material to control the mortality of white shrimp in traditional plus pond. This research was designed to use explorative experiment and experimental laboratory methods which used completed random sampling design. Collected data was analyzed with analysis of variance for examination of survival rate (SR), total haemocyte count (THC) and differensial haemocyte Count (DHC). The research divided into 2 part of riset: (1) Identification, cultivation Zoothamnium penaei, analysed of membrane protein by SDS-PAGE, (2) Field test protein membran on Survival Rate level, immune response (THC and/or DHC level) and infestation of Zoothamnium penaei in traditional plus pond. The result showed that there were seven bands membrane protein of Zoothamnium penaei with molecular weight 38 kDa, 48 kDa, 67 kDa, 71 kDa, 77 kDa, 98 kDa dan 104 kDa by using SDS-PAGE. Immunogenicity tested decrease by using ELISA and western blotting there are only found three bands with molecular weight 38 kDa, 48 kDa dan 67 kDa. The membrane protein could increase the immun respons and decrease the mortality, by subsequenly, it could increase the survival rate from 17% until 68% and pressured the parasite infestation of white shrimp.