Use of the phage display technique for detection of epitopes recognized by polyclonal rabbit gliadin antibodies

FEBS Letters

Thamm, Barbara²  Mothes, Thomas³  Uhlig, Holm³  Osman, Awad A³  Schneider-Mergener, Jens¹ 

[1] Institute of Medical Immunology of Humboldt-University Berlin, Schumann-Str. 20/21, 10117 Berlin, Germany;Core Unit B of Interdisciplinary Centre for Clinical Research of the University of Leipzig, Philipp-Rosenthal-Str. 55, 04103 Leipzig, Germany;Institute of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Paul-List-Str. 13–15, 04103 Leipzig, Germany

关键词: Phage display;    Peptide library;    Polyclonal antibody;    Gliadin;    Epitope;    Synthetic peptide;    CS;    consensus sequence(s);    gli/glu;    gliadin(s)/low molecular weight (LMW) glutenin(s);   

DOI  :  10.1016/S0014-5793(98)00887-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A random phage heptapeptide library was screened with rabbit antibodies against wheat flour proteins comprising gliadins and a small amount of low molecular weight glutenins (gli/glu). Gli/glu antibodies isolated from the sera selected different consensus sequences (CS). All CS contained tri- to pentapeptide stretches homologous to gli/glu sequences (proposed epitopes). In α- and γ-type gliadins, these sequences are clustered in the N-terminal region recently suspected to be toxic for humans with celiac disease. Peptides with CS were synthesized and checked for reactivity. Only immune and no control rabbit sera reacted with synthetic peptides. One of eight human sera containing gliadin antibodies was reactive as well (4/8 peptides) but control sera were negative. Thus the phage display technique is useful for epitope screening of polyclonal antibodies even in the case of a group of homologous but diverse antigens.

【 授权许可】

Unknown