Phage display as a promising approach for vaccine development

Journal of Biomedical Science

Review

Babak Bakhshinejad¹  Mehdi Yousefi²  Ali Aghebati-Maleki³  Morteza Motallebnezhad³  Hamid Nickho³  Behzad Baradaran⁴  Jafar Majidi⁴  Leili Aghebati-Maleki⁵ 

[1] Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran;Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;Immunology Research Center, Tabriz University of Medical sciences, Tabriz, Iran;Immunology Research Center, Tabriz University of Medical sciences, Tabriz, Iran;Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;Immunology Research Center, Tabriz University of Medical sciences, Tabriz, Iran;Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;

关键词: Bacteriophage;    Phage display;    Peptide library;    Vaccine development;    Biopanning;    Mimotope;   

DOI  :  10.1186/s12929-016-0285-9

 received in 2016-06-07, accepted in 2016-09-13,  发布年份 2016

来源: Springer

【 摘 要 】

Bacteriophages are specific antagonists to bacterial hosts. These viral entities have attracted growing interest as optimal vaccine delivery vehicles. Phages are well-matched for vaccine design due to being highly stable under harsh environmental conditions, simple and inexpensive large scale production, and potent adjuvant capacities. Phage vaccines have efficient immunostimulatory effects and present a high safety profile because these viruses have made a constant relationship with the mammalian body during a long-standing evolutionary period. The birth of phage display technology has been a turning point in the development of phage-based vaccines. Phage display vaccines are made by expressing multiple copies of an antigen on the surface of immunogenic phage particles, thereby eliciting a powerful and effective immune response. Also, the ability to produce combinatorial peptide libraries with a highly diverse pool of randomized ligands has transformed phage display into a straightforward, versatile and high throughput screening methodology for the identification of potential vaccine candidates against different diseases in particular microbial infections. These libraries can be conveniently screened through an affinity selection-based strategy called biopanning against a wide variety of targets for the selection of mimotopes with high antigenicity and immunogenicity. Also, they can be panned against the antiserum of convalescent individuals to recognize novel peptidomimetics of pathogen-related epitopes. Phage display has represented enormous promise for finding new strategies of vaccine discovery and production and current breakthroughs promise a brilliant future for the development of different phage-based vaccine platforms.

【 授权许可】

CC BY   
© The Author(s). 2016