Phosphatidylinositol transfer protein (PITPα) stimulates in vitro intra‐Golgi transport

FEBS Letters

Bogan, Andrew A¹  Waters, M.Gerard¹  Paul, Kimberly S¹ 

[1] Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA

关键词: Golgi apparatus;    Phosphatidylinositol transfer protein;    Vesicular transport;    Phospholipid;    Secretion;    BBC50;    50% ammonium sulfate precipitate of bovine brain cytosol;    DTT;    dithiothreitol;    EDTA;    ethylenediaminetetraacetic acid;    GlcNAc;    N-acetylglucosamine;    KPi;    potassium phosphate;    NSF;    N-ethylmaleimide-sensitive fusion protein;    PC;    phosphatidylcholine;    PCoA;    palmitoyl coenzyme A;    PI;    phosphatidylinositol;    PIP2;    phosphatidylinositol-4;    5-bisphosphate;    PITP;    phosphatidylinositol transfer protein;    PLC;    phospholipase C;    PVDF;    polyvinylidene difluoride;    SNAP;    soluble NSF attachment protein;    TGN;    trans-Golgi network;    VSV;    vesicular stomatitis virus;    Bd;    bound;    FT;    flow-through;    Ld;    load;   

DOI  :  10.1016/S0014-5793(98)00722-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a cell-free assay designed to reconstitute cis-to-medial intra-Golgi vesicular transport, we identified at least four crude activities in bovine brain cytosol that stimulate this assay. We have purified one of these activities to near homogeneity and have identified this M _r 36 kDa protein to be the α isoform of phosphatidylinositol transfer protein (PITPα) by N-terminal peptide sequencing, immunoreactivity with PITP-specific antisera, and the ability of recombinant PITPα to stimulate in vitro intra-Golgi transport. From these data, we conclude that in vitro Golgi transport is facilitated by PITPα.

【 授权许可】

Unknown