Molecular basis for low temperature compartment formation by transitional endoplasmic reticulum of rat liver

FEBS Letters

Cassagne, Claude¹  Juguelin, Hélène¹  Morré, D.James²  Moreau, Patrick¹ 

[1] Centre National de la Recherche Scientifigue, Institute de Biochimie Cellulaire et de Neurochimie, 33077 Bordeaux Cédex, France;Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA

关键词: Membrane traffic;    Low temperature compartment;    Golgi apparatus;    Phospholipid;    HEPES;    N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid);    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PI;    phosphatidylinositol;    PS;    phosphatidylserine;   

DOI  :  10.1016/0014-5793(92)81337-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular basis for temperature compartment formation was investigated using a cell-free system from rat liver. The donor was from liver slices prelabeled with [³H]acetate. Unlabeled Golgi apparatus membranes were immobilized on nitrocellulose as the acceptor. When transfer was determined as a function of temperature, a transition in transfer activity was observed at low temperatures (≤ 20°C) similar to that seen in vivo. The decrease in transfer efficiency correlated with a decrease in phosphatidylethanolamine and phosphatidylserine content of the transition vesicles formed. By adding lipid mixtures enriched in these lipids to the vesicles, their ability to fuse with the cis Golgi apparatus was reconstituted. These findings provide evidence for a role for lipids in low temperature compartment formation.

【 授权许可】

Unknown