Transport of apically but not basolaterally internalized ricin to the Golgi apparatus is stimulated by 8‐Br‐cAMP in MDCK cells

FEBS Letters

Sandvig, Kirsten¹  Llorente, Alicia¹  van Deurs, Bo² 

[1] Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway;Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark

关键词: Ricin;    Membrane traffic;    Endosome;    Golgi apparatus;    cAMP;    MDCK cell;    AKAPs;    A-kinase anchoring proteins;    BSA;    bovine serum albumin;    8-Br-cAMP;    8-bromoadenosine-3′;    5′-cyclic monophosphate;    DMEM;    Dulbecco's modified Eagle's medium;    H-89;    N-(2-[p-bromo cinnamylamino]-ethyl-5-isoquinolinesulfonamide;    HRP;    horseradish peroxidase;    MDCK;    Madin-Darby canine kidney;    PBS;    phosphate-buffered saline;    PKA;    protein kinase A;    PKC;    protein kinase C;    UDP-galactose;    uridine diphosphogalactose;   

DOI  :  10.1016/S0014-5793(98)00754-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The plant toxin ricin has to be transported to the Golgi apparatus after endocytosis to exert its toxic effect. In this study we show that transport of apically endocytosed ricin to the Golgi apparatus is stimulated by 8-Br-cAMP in polarized MDCK cells. This stimulation is counteracted by the PKA inhibitor H-89. In contrast, there is no increase in the transport to the Golgi apparatus of ricin internalized from the basolateral membrane. These results suggest that protein kinase A selectively regulates endosome to Golgi transport in these cells.

【 授权许可】

Unknown