Identification of two new μ‐adaptin‐related proteins, μ‐ARP1 and μ‐ARP2

FEBS Letters

Kilimann, Manfred W¹  Wang, Xiaolu¹ 

[1] Institut für Physiologische Chemie, Medizinische Fakultät, Ruhr-Universität Bochum, D-44780 Bochum, Germany

关键词: Membrane traffic;    Membrane vesicle;    Clathrin;    Coat protein;    Endocytosis;    Endosome;   

DOI  :  10.1016/S0014-5793(96)01500-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the cDNA cloning, primary structure and tissue distribution of two new proteins homologous to μ-adaptins, the medium chains of the clathrin coat adaptor complexes. Both predicted proteins share 60% amino acid sequence identity with each other and 27–31% identity with μ1-adaptin (ap47) and μ2-adaptin (ap50). Lower similarity (23–25% identity) is found with two other μ-adaptin-related proteins, p47A/B, and there is similarity over the N-terminal 150 amino acids with the adaptin small chains and δ-COP. The mRNAs of both molecules are expressed in all tissues analyzed, but with different profiles of relative abundance. μ-ARP1 is most abundant in brain, ovary and lung, whereas μ-ARP2 is prominently expressed in testis. These proteins suggest the existence of as yet uncharacterized types of clathrin- or non-clathrin-associated protein coats in cellular membrane traffic, of which they are probably prototype subunits, and provide molecular markers and probes for their characterization.

【 授权许可】

Unknown