Membrane phospholipid asymmetry in DMPC‐induced human red cell vesicles

FEBS Letters

Ott, Peter¹  de Jong, Kitty¹ 

[1] Institut für Biochemie und Molekularbiologie, Bühlstrasse 28, CH-3012 Bern, Switzerland

关键词: Phospholipid asymmetry;    Phospholipase A2;    Prothrombinase assay;    Erythrocyte;    Membrane vesicle;    Membrane skeleton;    DMPC;    1;    2-dimyristoyl;    -sn-glycero-3-phosphorylcholine;    PL;    phospholipid;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PS;    phosphatidylserine;    SM;    sphingomyelin;    PLA2;    phospholipase A2;    Hgb;    haemoglobin;    ATP;    adenosine 5′-triphosphate;    EDTA;    ethylene diamine tetraacetic acid;    HSA;    human serum albumin;    AChE;    acetylcholinesterase;   

DOI  :  10.1016/0014-5793(93)81708-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vesicles that do not contain spectrin were released from human erythrocytes by incubation with dimyristoylphosphatidylcholine. The transbilayer orientation of membrane phospholipids was subsequently determined by two independent methods. Incubation with phospholipase A₂ revealed that the phospholipid asymmetry observed in red blood cells was essentially preserved in vesicles. By use of the prothrombinase assay a still highly asymmetric distribution of phosphatidylserine could be demonstrated in spite of its slightly increased exposure on the vesicle surface. These results show that membrane phospholipid asymmetry can be maintained in a system that does not contain an intact membrane skeleton or spectrin.

【 授权许可】

Unknown