Measurement of the rotational behaviour of virus particles during adsorption to the host cell surface

FEBS Letters

Cherry, R.¹  Bayer, M.E.¹  Morrison, I.¹ 

[1] Institute for Cancer Research, Philadelphia, PA 19111, USA

关键词: Rotational diffusion;    Virus adsorption;    Membrane vesicle;    Transient dichroism;    Hydrodynamic diameter;    Virus immobilization;   

DOI  :  10.1016/0014-5793(84)80762-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rotational diffusion of bacteriophage ϵ15 was measured before and after virus adsorption to outermembrane vesicles of the host Salmonella anatum. The virus capsid was labeled with eosin isothiocyanate, and the decay of transient dichroism following dye excitation by pulses of plane-polarized light was measured. From the data, the rotational diffusion constant of the unadsorbed virion and its hydrodynamic diameter were estimated and found to be consistent with electron microscopic measurements of the capsid dimensions. Addition of outer membrane vesicles of S. anatum to the virus suspension revealed the immobilization of the virus particles on the membrane surface.

【 授权许可】

Unknown