Carbocyanine dyes used as fluorescent triplet probes for measuring slow rotational diffusion of lipids in membranes

FEBS Letters

Johnson, Pauline¹  Garland, Peter B.¹ 

[1] Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland

关键词: Carbocyanine dyes;    Fluorescent probe;    Triplet state;    Liposome;    Rotational diffusion;    Membrane;   

DOI  :  10.1016/0014-5793(83)80650-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A method for measuring the slow rotational diffusion of lipids or lipid domains in membranes has been developed. It covers the time range from 20μs–5 ms, and has a sensitivity > 5 × 10⁴ molecules of probe. The method uses acyl-substituted carbocyanine dyes as fluorescent triplet probes and a laser-microscope combination for excitation and measurement of the triplet state. Rotation rates in dimyristoylphosphatidylcholine vesicles were sensitive to the liquid-to-gel transition. Slow rotations with relaxation times of about 100μs were detected at the transition temperature region.

【 授权许可】

Unknown