FITC‐labeled lipopolysaccharide: use as a probe for liposomal membrane incorporation studies

FEBS Letters

Omel'yanenko, V.G.¹  Petrov, A.B.²  L'vov, V.L.³  Trubetskoy, V.S.¹  Dmitriev, B.A.³  Torchilin, V.P.¹  Koshkina, N.V.¹ 

[1]USSR Cardiology Research Center, Moscow, USSR

[2]Mechnikov Research Institute for Vaccines and Sera, Moscow, USSR

[3]Gamaleya Institute of Epidemiology and Microbiology, Moscow, USSR

关键词: Lipopolysaccharide;    Liposome;    Toxicity;    Fluorescent probe;    Fluorescence polarization;    LPS;    lipopolysaccharide;    FITC;    fluorescein isothiocyanate;    DRV;    dehydration/rehydration vesicles;    DPH;    1;    6-diphenylhexatriene;    AD;    actinomycin D;   

DOI  :  10.1016/0014-5793(90)81123-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxirity decrease in actinomycin D-sensitized mice. Fluorescence intensity increase was observed upon the insertion of FITC-LPS into the membrane of dehydration/rehydration vesicles and vesicles obtained by co-sonication of lipid suspension and FITC-LPS. Following FITC-LPS fluorescence polarization it was shown that the substance seems to be clusterized m the liposomal membrane starting from FITC-LPS/lipid molar ratio 1:800.

【 授权许可】

Unknown