The antioxidant action of 2‐methyl‐6‐(p‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐α]pyrazin‐3‐one (MCLA), a chemiluminescence probe to detect superoxide anions

FEBS Letters

Tampo, Yoshiko¹  Yonaha, Masanori¹  Tsukamoto, Maki¹ 

[1]Division of Environmental Hygiene, Hokkaido College of Pharmacy, 7-1 Katsuraoka-cho, Otaru, Hokkaido 047-0264, Japan

关键词: 2-methyl-6-(p-methoxyphenyl)-3;    7-dihydroimidazo[1;    2-α]pyrazin-3-one;    Chemiluminescence;    Lipid peroxidation;    Antioxidant;    Superoxide anion;    Free radical;    AAPH;    2;    2′-azobis (2-amidinopropane) dihydrochloride;    CHP;    cumene hydroperoxide;    DPPH;    1;    1-diphenyl-2-picrylhydrazyl;    MCLA;    2-methyl-6-(p-methoxyphenyl)-3;    7-dihydroimidazo[1;    2-α]pyrazin-3-one;    MDA;    malondialdehyde;    PC(A);    l-α-phosphatidylcholine β-arachidonoyl γ-stearoyl;    PC(DP);    l-α-phosphatidylcholine dipalmitoyl;    PPi;    pyrophosphate;    PS;    dl-α-phosphatidyl-l-serine dipalmitoyl;    SOD;    superoxide dismutase;    TBARS;    thiobarbituric acid-reactive substances;   

DOI  :  10.1016/S0014-5793(98)00689-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The antioxidant effect of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-α]pyrazin-3-one (MCLA), a Cypridina luciferin analog that acts as a chemiluminescence probe to detect O^⋅− ₂, was investigated. MCLA produced a lag in oxygen consumption induced by cumene hydroperoxide in microsomes or by 2,2′-azobis (2-amidinopropane) dihydrochloride in liposomes and disappeared during the duration of the lag. MCLA profoundly inhibited the propagation reaction in Fe²⁺-dependent lipid peroxidation in liposomes, and MCLA disappearance accompanied by suppression of oxygen consumption markedly occurred in liposomes susceptible to peroxidation. Thiobarbituric acid-reactive substances in all systems used were also suppressed by MCLA dose dependently. These results indicate that MCLA has an antioxidant property through scavenging free radicals.

【 授权许可】

Unknown