Possible involvement of superoxide anion in the induction of cyanide‐resistant respiration in Hansenula anomala

FEBS Letters

Sakajo, Shigeru²  Yoshimoto, Akio²  Koga, Satoshi¹  Nakano, Minoru¹  Minagawa, Nobuko² 

[1] Photon Medical Center, Hamamatsu University School of Medicine, Hamamatsu, Japan;Department of Biochemistry, Niigata College of Pharmacy, 5-13-2 Kamishin'ei-cho, Niigata 950-21, Japan

关键词: Mitochondria;    Superoxide anion;    Chemiluminescence;    Cyanide-resistant respiration;    MCLA;    2-methyl-6-(p-methoxyphenyl)-3;    7-dihydroimidazo[1;    2-a]pyrazin-3-one;    SOD;    superoxide dismutase;    BHA;    butylated hydroxyanisole;    BHT;    butylated hydroxytoluene;   

DOI  :  10.1016/0014-5793(92)80444-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A chemiluminescence study showed that Q_i site inhibitors such as antimycin A induce O₂ ⁻ generation in respiring cyanide-sensitive mitochondria from the yeast, Hansenula anomala. The O₂ ⁻ generation was suppressed by radical scavengers such as flavone, butylated hydroxyanisole, and Co₀. Induction of cyanide-resistant respiration in H. anomala cells by Q_i site inhibitors was also inhibited by these radical scavengers. Furthermore, antimycin A-induced synthesis of the mitochondrial 36-kDa protein, which is thought to be the alternative oxidase functional in the cyanide-resistant respiratory pathway, was abolished by the addition of flavone. These observations suggest that O₂ ⁻ is somehow involved in the induction of cyanide-resistant respiration.

【 授权许可】

Unknown