1,2‐Diacylglycerol hydroperoxides induce the generation and release of superoxide anion from human polymorphonuclear leukocytes

FEBS Letters

Ito, Takashi²  Kambayashi, Yasuhiro¹  Nakano, Minoru¹  Yamamoto, Yorihiro³ 

[1] Department of Photon and Free Radical Research, Japan Immunoresearch Laboratories, 351-1 Nishiyokote Cho, Takasaki 370, Japan;Department of Neurology, Tokyo Metropolitan Matsuzawa Hospital, Kamikitazawa, Setagaya-ku, Tokyo 156, Japan;Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153, Japan

关键词: Diacylglycerol hydroperoxide;    Human polymorphonuclear leukocyte;    Superoxide anion;    2-Methyl-6-(p-methoxyphenyl)-3;    7-dihydroimidazo[1;    2-a]pyrazin-3-one;    Protein kinase C;    PKC;    protein kinase C;    PMA;    phorbol 12-myristate 13-acetate;    1;    2-LLG-OOH;    1;    2-dilinoleoylglycerol hydroperoxide;    1;    2-LLG-OOH;    1;    2-dilinoleoylglycerol hydroxide;    1;    2-DAG-OOH;    1;    2-diacylglycerol hydroperoxide;    1;    2-DAG-OH;    1;    2-diacylglycerol hydroxide;    PMNs;    polymorphonuclear leukocyte(s);    MCLA;    2-methyl-6-(p-methoxyphenyl)-3;    7-dihydroimidazo[1;    2-a]pyrazin-3-one;    PLPC;    1-palmitoyl-2-linoleoylphosphatidylcholine;    PLPC-OOH;    PLPC hydroperoxide;    PLPC-OH;    PLPC hydroxide;    1;    3-LLG;    1;    3-dilinoleoylglycerol;    1;    3-LLG-OOH;    1;    3-LLG hydroperoxide;    SOD;    superoxide dismutase;    PLG;    1-palmitoyl-2-linoleoylglycerol;    PLG-OOH;    PLG hydroperoxide;    PLG-OH;    PLG hydroxide;    HBSS;    Hanks' balanced salt solution;    HPLC;    high performance liquid chromatography;    MLI;    maximal luminescence intensity;    cpm;    counts/min;   

DOI  :  10.1016/S0014-5793(97)00823-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined the effect of 1-palmitoyl-2-linoleoylglycerol (PLG), PLG hydroxide (PLG-OH), and PLG hydroperoxide (PLG-OOH) on the release of superoxide anion from human PMNs monitored by the chemiluminescence generated by the superoxide anion-sensitive reagent, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA). PLG-OOH at low micromolar concentrations stimulated human PMNs whereas PLG and PLG-OH did not. 1,3-Dilinoleoylglycerol hydroperoxide, 1-palmitoyl-2-linoleoylphosphatidylcholine hydroperoxide, and linoleic acid hydroperoxide were much less efficient in stimulating human PMNs than PLG-OOH. The PKC inhibitors, chelerythrine chloride and staurosporine, inhibited the stimulation of PMNs. Possible pathophysiological role of 1,2-diacylglycerol hydroperoxides is discussed.

【 授权许可】

Unknown