Chemiluminescence determination of hydroperoxides following radiolysis and photolysis of free amino acids

FEBS Letters

Bevan, Ruth¹  Lunec, Joseph¹  Griffiths, Helen²  Robinson, Stephen¹ 

[1] Division of Chemical Pathology, Centre for Mechamisms of Human Toxicity, MRC Toxicology Unit, University of Leicester, PO Box 138, Lancaster Road, Leicester, LE1 9HN, UK;Pharmaceutical Sciences, Aston Triangle, Aston University, Birmingham, B4 7ET, UK

关键词: Free radical;    Hydroperoxide;    Chemiluminescence;    Amino acid;    Gamma radiolysis;    UVB radiation;   

DOI  :  10.1016/S0014-5793(98)00679-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hydroperoxides were determined in selected amino acids using three free radical generating systems by a sensitive (50 pmol limit of detection) and specific high performance liquid chromatography (HPLC)/chemiluminescence method. UVB and gamma radiation produced significant hydroperoxide formation, particularly in the aromatic amino acids tyrosine and tryptophan. Hydroperoxide yield was found to be dependent on both amino acid and irradiation source. Generation of hydrogen peroxide as a by-product of irradiation caused interference with chemiluminescence detection demonstrating the need for catalase addition. Hydroperoxides were not detectable following metal-catalysed H₂O₂ breakdown. We suggest that metal ions could interfere with the detection of hydroperoxides by causing preferential decomposition.

【 授权许可】

Unknown