Major differences in oxysterol formation in human low density lipoproteins (LDLs) oxidized by OH/O2− free radicals or by copper

FEBS Letters

Bonnefont-Rousselot, Dominique³  Beaudeux, Jean-Louis³  Thérond, Patrice²  Gardès-Albert, Monique¹  Zarev, Stéphanie³  Legrand, Alain³ 

[1] Laboratoire de Chimie-Physique, URA 400 CNRS, 45, rue des Saints-Pères, 75270 Paris Cedex 06, France;Laboratoire de Biochimie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin Bicêtre Cedex, France;Laboratoire de Biochimie Métabolique et Clinique, Faculté de Pharmacie, 4, avenue de l'Observatoire, 75270 Paris Cedex 06, France

关键词: Copper;    Free radical;    Hydroperoxide;    Lipoprotein;    Low density lipoprotein;    Oxidation;    Oxysterol;    CD;    conjugated dienes;    CE;    cholesteryl ester;    CEOOH;    cholesteryl ester hydroperoxide;    5;    6β-epoxy;    5;    6β-epoxycholesterol;    7-OH;    7β-hydroxycholesterol;    HPETE;    hydroperoxyeicosatetraenoic acid;    EDTA;    ethylene diamine tetraacetic acid;    HPLC;    high performance liquid chromatography;    7-KC;    7-ketocholesterol;    LDL;    low density lipoprotein;    PUFA;    polyunsaturated fatty acid;    TBARS;    thiobarbituric acid-reactive substances;   

DOI  :  10.1016/S0014-5793(99)00564-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aim of our study was to determine the oxysterol formation in low density lipoproteins (LDLs) oxidized by defined oxygen free radicals (OH/O₂ ⁻). This was compared to the oxysterol produced upon the classical copper oxidation procedure. The results showed a markedly lower formation of oxysterols induced by OH/O₂ ⁻ free radicals than by copper and thus suggested a poor ability of these radicals to initiate cholesterol oxidation in LDLs. Moreover, the molecular species of cholesteryl ester hydroperoxides produced by LDL copper oxidation seemed more labile than those formed upon OH/O₂ ⁻-induced oxidation, probably due to their degradation by reaction with copper ions.

【 授权许可】

Unknown