Hepatic apolipoprotein B synthesis in copper‐deficient rats

FEBS Letters

Mazur, Andrzej¹  Gueux, Elyett¹  Rayssiguier, Yves¹  Sérougne, Colette²  Nassir, Fatiha¹ 

[1] Laboratoire des Maladies Métaboliques, INRA, Theix, 63122 St. Génes Champanelle, France;Laboratoire de Physiologie de la Nutrition, Université Paris Sud, 91405 Orsay Cédex, France

关键词: Copper;    Apolipoprotein B;    Lipoprotein;    Liver;    Rat;    apo;    apolipoprotein;    CM;    chylomicrons;    EDTA;    ethylenediamine tetraacetic acid;    ELISA;    enzyme-linked immunoassay;    HDL;    high density lipoproteins;    LDL;    low density lipoproteins;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phosphate-buffered saline;    PMSF;    phenyl methyl sulfonyl fluoride;    SDS;    sodium dodecyi sulfate;    TCA;    trichloroacetic acid;    VLDL;    very low density lipoproteins;   

DOI  :  10.1016/0014-5793(93)81105-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present study was designed to examine if induction of apolipoprotein B synthesis is associated with hypercholesterolemia in copper-deficient rats. This hypercholesterolemia mainly resides in an increase in the HDL-1 and LDL and is associated with a significant increase in plasma apoB concentration. Liver apoB mRNA levels were not significantly modified in deficient animals as compared to control rats. Studies on liver apolipoprotein synthesis indicated that apoB100 synthesis was increased in deficient animals whereas apoB48 synthesis was unchanged. Thus, it appears that the increase in apoB synthesis in the liver of copper-deficient rats occurs at the posttranscriptional level. The selective increase in apoB100 synthesis indicates the possible impact of this deficiency on the editing of apoB. An increase in apoB100 synthesis by the liver in copper-deficient rats may significantly contribute to the increase in plasma concentration of LDL.

【 授权许可】

Unknown