Human/mouse interleukin‐1 receptor/receptor accessory protein interactions in IL‐1β‐induced NFκB activation

FEBS Letters

Bartfai, Tamas²  Lundkvist, Johan²  Layé, Sophie¹ 

[1] INSERM U394, rue C. Saint-Saëns, 33077 Bordeaux, France;Department of Neurochemistry and Neurotoxicology, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Human interleukin-1 receptor type I;    Mouse interleukin-1 receptor accessory protein;    Nuclear factor κB;    Heteroduplex;    293-hIL-1RI/mIL-1RAcP;    293 cells transfected with hIL-1RI and mIL-1RAcP cDNAs;    P2;    anti-hIL-1RI;    4C5;    anti-mIL-1RAcP;    35F5;    anti-mIL-1RI;    4E2;    anti-mIL-1RII;    C127-hIL-1RI;    C127 cells transfected with human IL-1RI;    HEK;    human embryonic kidney;    IL-1RI;    IL-1 receptor type I;    IL-1RAcP;    IL-1R accessory protein;    IL-1;    interleukin-1;    m;    murine;    h;    human;    NFκB;    nuclear factor κB;   

DOI  :  10.1016/S0014-5793(98)00537-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined whether functional heterologous complexes between human IL-1RI (hIL-1RI) and murine IL-1R accessory protein (mIL-1RAcP) can be formed, utilizing human fibroblast HEK 293 cells and murine fibroblast C127 cells, non-transfected or stably transfected with hIL-1RI (C127-hIL-1RI), respectively. In non-transfected C127 cells, IL-1β signalled through the mIL-1RI-mIL-1RAcP complex and activated NFκB p50/p65 heterodimers. In C127-hIL-1RI cells, IL-1β signalled through the hIL-1RI and activated both p65/p65 and p50/p65 NFκB complexes, where only the activation of NFκB p65/p65 was dependent on mIL-1RAcP. Thus, clearly both homologous and heterologous IL-1RI-IL-1RAcP interactions support NFκB translocation, but with differences in signalling pattern.

【 授权许可】

Unknown