Sequence‐dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H

FEBS Letters

Iwai, Shigenori¹  Inoue, Hideo¹  Ohtsuka, Eiko¹  Hayase, Yoji¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: 2′-O-Methyloligonucleotide;    Chimeric oligonucleotide;    Heteroduplex;    RNase H;    Site-specific cleavage;    RNA hydrolysis;   

DOI  :  10.1016/0014-5793(87)80171-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo- and 2′-O-methylnucleosides, a nonaribonucleotide, [5′-³² pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3′m(UG)d(AATG)m(GAC)5′ was used as a hybridization strand, *pACUUACCUG was cleaved between C6 and C7 to yield *pACUUAC. In the presence of 3′m(UGAA)d(TGGA)m(C)5′, the nonaribonucleotide was hydrolyzed, mainly between U8 and C9, to give *pACUUACCU. This method will have a variety of applications in the field of RNA engineering.

【 授权许可】

Unknown