Yeast ribonuclease H(70) cleaves RNA‐DNA junctions

FEBS Letters

Karwan, Robert¹  Wintersberger, Ulrike¹ 

[1] Department of Molecular Genetics, Institute for Tumorbiology and Cancer Research, University of Vienna, Borschkegasse 8a, A-1090 Wien, Austria

关键词: RNase H;    DNA-RNA hybrid;    DNA replication;    RNA primer removal;    (Saccharomyces cerevisiae);   

DOI  :  10.1016/0014-5793(86)80978-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A specific substrate, M 13 DNA:RNA-[³²P]DNA, was synthesized to investigate the mode of cleavage of enzymes with RNase H activity. RNase H(70) from Saccharomyces cerevisiae hydrolyzes the phosphodiester bond at the RNA-DNA junction of this substrate, thereby producing a 5'-monophosphate-terminated polydeoxyribonucleotide and 3'-hydroxyl-terminated oligoribonucleotides.

【 授权许可】

Unknown