Activation of NF‐κB in human neutrophils during phagocytosis of bacteria independently of oxidant generation

FEBS Letters

Winterbourn, Christine C.¹  Vollebregt, Marieke¹  Hampton, Mark B.¹ 

[1]Department of Pathology, Christchurch School of Medicine, P.O. Box 4345, Christchurch, New Zealand

关键词: Nuclear factor κB;    Neutrophil;    Phagocytosis;    Hydrogen peroxide;    Staphylococcus aureus;   

DOI  :  10.1016/S0014-5793(98)00821-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have exposed human neutrophils to opsonized Staphylococcus aureus and used an electrophoretic mobility shift assay to show activation of the transcription factor NF-κB above basal levels. Activation was evident within 10 min and was increased with higher bacteria:neutrophil ratios. The neutrophil NADPH oxidase inhibitor diphenylene iodonium, catalase, and other oxidant scavengers did not inhibit NF-κB activation, and no activation was seen with added hydrogen peroxide. Oxidants produced during phagocytosis, therefore, are not involved in the activation mechanism.

【 授权许可】

Unknown