Kinetics of nitric oxide and hydrogen peroxide production and formation of peroxynitrite during the respiratory burst of human neutrophils

FEBS Letters

Catz, Sergio D.¹  Boveris, Alberto¹  Pargament, Griselda A.¹  Carreras, Maria Cecilia¹  Poderoso, Juan J.¹ 

[1] Laboratory of Oxygen Metabolism, University Hospital, University of Buenos Aires, Cordoba 2351, 1120 Buenos Aires, Argentina

关键词: Neutrophil;    Nitric oxide;    Peroxynitrite;    Superoxide anion;    Hydrogen peroxide;    Respiratory burst;   

DOI  :  10.1016/0014-5793(94)80241-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitric oxide (^.NO) release, oxygen uptake and hydrogen peroxide (H₂O₂) production elicited by increasing phorbol 12-myristate 13-acetate (PMA) concentrations were measured in human neutrophils. Half-maximal activities were sequentially elicited at about 0.0001–0.001 μg (^.NO) and 0.001-0.01 μg (H₂O₂). At saturated PMA concentrations, ^.NO production, oxygen uptake and H₂O₂ release were 0.56 ± 0.04, 3.32 ± 0.52 and 1.19±0.17 nmol · min⁻¹ · 10⁶ cells⁻¹. ^.NO production accounts for about 30% of the total oxygen uptake. Luminol-dependent chemiluminescence, reported to detect NO reactions in other inflammatory cells, was also half-maximally activated at about 0.001-0.01 μg . Preincubation with N ^G-monomethyl-l-arginine (l-NMMA) decreased O₂ uptake and ^.NO release but increased H₂O₂ production, while superoxide dismutase (SOD) increased ^.NO detection by 30%. Chemiluminescence was also reduced by preincubation with l-NMMA and/or SOD. The results indicate that ^.NO release is part of the integrated response of stimulated human neutrophils and that, in these cells, kinetics of ″NO and O₂ ^.− release favour the formation of other oxidants like peroxynitrite.

【 授权许可】

Unknown