FEBS Letters | |
Kinetics of nitric oxide and hydrogen peroxide production and formation of peroxynitrite during the respiratory burst of human neutrophils | |
Catz, Sergio D.1  Boveris, Alberto1  Pargament, Griselda A.1  Carreras, Maria Cecilia1  Poderoso, Juan J.1  | |
[1] Laboratory of Oxygen Metabolism, University Hospital, University of Buenos Aires, Cordoba 2351, 1120 Buenos Aires, Argentina | |
关键词: Neutrophil; Nitric oxide; Peroxynitrite; Superoxide anion; Hydrogen peroxide; Respiratory burst; | |
DOI : 10.1016/0014-5793(94)80241-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Nitric oxide (.NO) release, oxygen uptake and hydrogen peroxide (H2O2) production elicited by increasing phorbol 12-myristate 13-acetate (PMA) concentrations were measured in human neutrophils. Half-maximal activities were sequentially elicited at about 0.0001–0.001 μg (.NO) and 0.001-0.01 μg (H2O2). At saturated PMA concentrations, .NO production, oxygen uptake and H2O2 release were 0.56 ± 0.04, 3.32 ± 0.52 and 1.19±0.17 nmol · min−1 · 106 cells−1. .NO production accounts for about 30% of the total oxygen uptake. Luminol-dependent chemiluminescence, reported to detect NO reactions in other inflammatory cells, was also half-maximally activated at about 0.001-0.01 μg . Preincubation with N G-monomethyl-l-arginine (l-NMMA) decreased O2 uptake and .NO release but increased H2O2 production, while superoxide dismutase (SOD) increased .NO detection by 30%. Chemiluminescence was also reduced by preincubation with l-NMMA and/or SOD. The results indicate that .NO release is part of the integrated response of stimulated human neutrophils and that, in these cells, kinetics of ″NO and O2 .− release favour the formation of other oxidants like peroxynitrite.
【 授权许可】
Unknown
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