Lysophosphatidylcholine generates superoxide anions through activation of phosphatidylinositol 3‐kinase in human neutrophils

FEBS Letters

Horiuchi, Hisanori¹  Kita, Toru¹  Arai, Hidenori¹  Nishioka, Hiroaki¹ 

[1] Department of Geriatric Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan

关键词: Lysophosphatidylcholine;    Superoxide anion;    Phosphatidylinositol 3-kinase;    Protein kinase C;    Neutrophil;    LPC;    lysophosphatidylcholine;    O⋅− 2;    superoxide anions;    PI3 kinase;    phosphatidylinositol 3-kinase;    PKC;    protein kinase C;    PC;    phosphatidylcholine;    PMA;    phorbol 12-myristate 13-acetate;    fMLP;    formyl-methionyl-leucyl-phenylalanine;    SOD;    superoxide dismutase;    OD;    optical density;    PBS;    phosphate-buffered saline;    FACS;    fluorescence-activated cell sorter;    PI;    phosphatidylinositol;    LPA;    lysophosphatidic acid;    PI3P;    phosphatidylinositol 3-phosphate;    LDL;    low density lipoprotein;   

DOI  :  10.1016/S0014-5793(98)01526-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lysophosphatidylcholine (LPC) accumulates in inflammatory tissues, where neutrophils are recruited to generate superoxide anions (O^⋅− ₂). Here, we show that LPC stimulates O^⋅− ₂ generation in human neutrophils and that the activity is inhibited with phosphatidylinositol 3-kinase (PI3 kinase) inhibitors, but not with protein kinase C (PKC) inhibitors. Furthermore, we demonstrate that LPC activates PI3 kinase in neutrophils. Thus, LPC might contribute to host defense by generating O^⋅− ₂ in neutrophils through PI3 kinase activation, but not through PKC activation.

【 授权许可】

Unknown