FEBS Letters | |
Metabolism of 8‐iso‐prostaglandin F2α | |
Basu, S1  | |
[1] Department of Geriatrics, Faculty of Medicine, Uppsala University, S-751 25 Uppsala, Sweden | |
关键词: Metabolism; Isoprostane; 8-iso-Prostaglandin F2α; Eicosanoid; Pharmacokinetics; Lipid peroxidation; GC-MS; gas chromatography-mass spectrometry; RIA; radioimmunoassay; TMS; trimethylsilyl; BSTFA; bis(trimethylsilyl)trifluroacetamide; HPLC; high pressure liquid chromatography; RP; reversed-phase; SP; straight-phase; i.v.; intravenous; PG; prostaglandin; 15-PGDH; 15-hydroxy prostaglandin dehydrogenase; | |
DOI : 10.1016/S0014-5793(98)00481-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Tritium labelled (1.1 MBq/17.7 μg/kg) and unlabelled 8-iso-PGF2α (43 μg/kg) were administered intravenously to female rabbits and frequent blood and continuous urinary samples were collected up to 4 h. The total radioactivity was lost rapidly from the circulation. About 80% of the total radioactivity was found in urine within 4 h. The plasma half-life of 8-iso-PGF2α is found to be 1 min at the distribution phase. The terminal elimination phase half-life was about 4 min. At 1.5 min after administration 64%, 19% and 13% of the plasma radioactivity represented 8-iso-PGF2α, 15-keto-8-iso-PGF2α and β-oxidised products, respectively. The values for 20-min plasma were 5%, 2% and 88%. The radiochromatograms from 10 min–4 h urinary samples were dominated by more polar β-oxidised products. α-Tetranor-15-keto-13,14-dihydro-8-iso-PGF2α was identified as a major urinary metabolite.Thus, 8-iso-PGF2α metabolises in the rabbit mainly to several degraded polar metabolites through dehydrogenation at C-15, reduction of Δ13-double bond and β-oxidation, and excretes efficiently into the urine.
【 授权许可】
Unknown
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