Urinary isoprostane excretion is not confounded by the lipid content of the diet

FEBS Letters

Tavazzi, I¹  Guidoux, R¹  Métairon, S¹  Fay, L.B¹  Turini, M.E¹  Richelle, M¹ 

[1] Nestlé Research Center, P.O. Box 44, 1000 Lausanne 26, Switzerland

关键词: Metabolism;    Isoprostane;    8-iso-Prostaglandin F2α;    Lipid peroxidation;    Malondialdehyde;    PUFA;    polyunsaturated fatty acid;    GC;    gas chromatography;    MS;    mass spectrometry;    MDA;    malondialdehyde;    SPE;    solid phase extraction;    PFB;    pentafluorobenzyl;    TLC;    thin layer chromatography;    TMS;    trimethylsilyl;   

DOI  :  10.1016/S0014-5793(99)01259-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study aims to determine if isoprostanes accurately reflect in vivo lipid peroxidation or whether they are influenced by the lipid content of the diet. Isoprostanes were measured in urine of healthy subjects under different conditions of lipid intake and under conditions of oxidative stress (fasting). We found that isoprostanes were not influenced by the lipid content of the diet: the urinary level remained constant over 24 h as well as over 4 consecutive days when switching from high to low lipid intake. Urinary isoprostane excretion was increased by 40% following a 24 h fast. We concluded that urinary isoprostane excretion reflects endogenous lipid peroxidation in vivo.

【 授权许可】

Unknown