Development of a novel biomarker of free radical damage in reperfusion injury after cardiac arrest

FEBS Letters

Liu, X.L.²  Rubertsson, S.²  Wiklund, L.²  Basu, S.¹  Nozari, A.² 

[1] Department of Geriatrics, Faculty of Medicine, Uppsala University, P.O. Box 609, SE-751 25 Uppsala, Sweden;Department of Anaesthesiology and Intensive Care, Faculty of Medicine, Uppsala University, Box 609, SE-751 25 Uppsala, Sweden

关键词: Isoprostane;    Prostaglandin;    Inflammation;    Oxidative stress;    Brain;    Cardiac arrest;    COX;    cyclooxygenase;    PG;    prostaglandin;    8-iso-PGF2α;    8-iso-prostaglandin F2α;    15-keto-dihydro-PGF2α (15-K-DH-PGF2α);    15-keto-13;    14-dihydro-prostaglandin F2α;    PGF2α;    prostaglandin F2α;    RIA;    radioimmunoassay;    ROSC;    restoration of spontaneous circulation;    CPR;    cardiopulmonary resuscitation;    PA;    pulmonary artery;    VF;    ventricular fibrillation;   

DOI  :  10.1016/S0014-5793(00)01279-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In a porcine model of cardiopulmonary resuscitation (CPR), we investigated changes in the plasma levels of 8-iso-PGF_2α, a marker for oxidative injury, and 15-keto-dihydro-PGF_2α, an inflammatory response indicator during the post-resuscitation period after cardiac arrest. Twelve piglets were subjected to either 2 or 5 min (VF2 and VF5 group) of ventricular fibrillation (VF) followed by 5 min of closed-chest CPR. Six piglets without cardiac arrest were used as controls. In VF5 group, 8-iso-PGF_2α in the jugular bulb plasma (draining the brain) increased four-fold. Jugular bulb 8-iso-PGF_2α in the control group remained unchanged. The 15-keto-dihydro-PGF_2α also increased four-fold in the VF5 group. Thus, 8-iso-PGF_2α and 15-keto-dihydro-PGF_2α measurements in jugular bulb plasma may be used as biomarkers for quantification of free radical catalyzed oxidative brain injury and inflammatory response in reperfusion injury.

【 授权许可】

Unknown