Biomarkers of free radical injury during spinal cord ischemia

FEBS Letters

Hellberg, A.³  Karacagil, S.³  Westman, J.²  Basu, S.¹  Ulus, A.T.³ 

[1] Sections of Geriatrics and Clinical Nutrition Research, Faculty of Medicine, Uppsala University, P.O. Box 609, SE-751 25 Uppsala, Sweden;Department of Cell Biology, Faculty of Medicine, Uppsala University, SE-751 23 Uppsala, Sweden;Department of Surgery, Faculty of Medicine, Uppsala University, SE-751 85 Uppsala, Sweden

关键词: Ischemia-reperfusion;    Prostaglandin;    Isoprostane;    Spinal cord;    Oxidative injury;    Inflammation;    PG;    prostaglandin;    TXB2;    thromboxane B2;    CSF;    cerebrospinal fluid;    DAXC;    distal aortic cross-clamping;    COX;    cyclooxygenase;   

DOI  :  10.1016/S0014-5793(01)02998-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasma and urinary levels of 8-iso-PGF_2α and 15-keto-dihydro-PGF_2α were analysed at baseline and during the ischemia-reperfusion period in experimental spinal cord ischemia. A significant and immediate increase of 8-iso-PGF_2α in plasma at the start and up to 60 min, and in the urine at 90–150 min following ischemia indicate an association of oxidative injury. The inflammatory response indicator 15-keto-dihydro-PGF_2α in plasma increased significantly at the start and up to 60 min after ischemia. No such increase was seen in animals with no spinal cord ischemia. Thus, free radical mediated and cyclooxygenase catalysed products of arachidonic acid are increased during spinal cord ischemia as a consequence of oxidative injury and inflammation.

【 授权许可】

Unknown