Retinol‐induced secretion of human retinol‐binding protein in yeast

FEBS Letters

Reppe, Sjur²  Smeland, Sigbjørn¹  Blomhoff, Rune¹  Moskaug, Jan ع 

[1] Institute for Nutrition Research, University of Oslo, P.O. Box 1046, Blindern, 0316 Oslo, Norway;Institute for Medical Biochemistry, University of Oslo, P.O. Box 1112, Blindern, 0317 Oslo, Norway

关键词: Retinol-binding protein;    Secretion;    Yeast;    hRBP;    human retinol-binding protein;    TTR;    transthyretin;    4-HPR;    N-(4-hydroxyphenyl)retinamide;    ER;    endoplasmic reticulum;    HepG2;    hepatoma G2;    PAGE;    polyacrylamide gel electrophoresis;    DTT;    dithiothreitol;    PVDF;    polyvinyl difluoride;    TCA;    trichloroacetic acid;    RA;    all-trans retinoic acid;    RAL;    all-trans retinal;    ROH;    all-trans retinol;    2-MEtOH;    β-mercaptoethanol;    EtOH;    ethanol;   

DOI  :  10.1016/S0014-5793(98)00428-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Retinol-binding protein (RBP) functions as a transporter for retinol (vitamin A) in plasma in higher eukaryotes. We have successfully expressed human RBP in Saccharomyces cerevisiae, and its secretion was found to be induced by retinol also in this lower eukaryote. Reduced induction of secretion by retinol in a temperature-sensitive sec18-1 mutant that is blocked in secretion at the restricted temperature suggests that as in mammalian cells, RBP can be released from the endoplasmic reticulum upon addition of retinol. Thus, the molecular mechanism involved in retinol-dependent secretion of RBP appears to be conserved in yeast, and this points to yeast as a putative model system for studying retinol-regulated secretion of RBP. RBP purified from yeast was found to be indistinguishable from RBP purified from human plasma in several functional assays.

【 授权许可】

Unknown