Interaction of Sec4 with GDI proteins from bovine brain, Drosophila melanogaster and Saccharomyces cerevisiae

FEBS Letters

Garrett, Michelle D.⁴  Novick, Peter J.⁴  Takai, Yoshimi¹  Kabcenell, Alisa K.³  Cheney, Clarissa M.²  Zahner, Joseph E.²  Kaibuchi, Kozo¹  Sasaki, Takuya¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan;Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA;Department of Biochemistry, Boehringer Ingelheim Pharmaceuticals, Ridgefield, CT 06877, USA;Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA

关键词: Sec4;    GDP dissociation inhibitor (GDI);    Secretion;    GTP-binding protein;    Exchange protein;    Yeast;    Sec4;    the protein product of the SEC4 gene;    KPi;    potassium phosphate;    DTT;    dithiothreitol;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    GTPγS;    guanosine 5'-O-thiotriphosphate;   

DOI  :  10.1016/0014-5793(93)80343-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rab GDP dissociation inhibitor (Rab GDI), will induce the dissociation of GDP-bound rab3A from synaptic membranes and will inhibit GDP dissociation from Sec4, a member of the Rab subgroup of the Ras GTPase superfamily which is required for exocytosis in Saccharomyces cerevisiae. We report that Rab GDI releases GDP-bound Sec4 from yeast membranes. dGDI, a Drosophila homologue can similarly inhibit GDP dissociation from Sec4 and release GDP-bound Sec4 from yeast membranes. An activity partially purified from yeast cytosol dissociates GDP-bound Sec4 from yeast membranes, suggesting that yeast also possess a GDI protein that functions to recycle Sec4 from its target membrane.

【 授权许可】

Unknown