Synthesis and secretion of bacterial α‐amylase by the yeast Saccharomyces cerevisiae

FEBS Letters

Luzikov, Y.N.¹  Kovaleva, I.E.¹  Novikova, L.A.¹ 

[1] Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Amylase;    α-;    Yeast;    Secretion;    Glycosylation;    Bacteria;   

DOI  :  10.1016/0014-5793(89)81451-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Amylase from Bacillus amyloliquefaciens, synthesized in yeast Saccharomyces cerevisiae without substitution of the signal sequence, is efficiently secreted from yeast cells: 60–70% of the overall amount of the enzyme is found in the culture fluid. In contrast to many yeast secretory proteins, which accumulate in the periplasmic space and in the cell wall, intracellular α-amylase is localized mainly in the cytoplasm. Obviously, transfer across the cell wall is not a rate-limiting step in α-amylase export from the cell. The glycosylated forms of proteins are predominantly found both inside the cell and in the culture medium.

【 授权许可】

Unknown