Phospholamban domain I/cytochrome b 5 transmembrane sequence chimeras do not inhibit SERCA2a

FEBS Letters

Kimura, Yoshihiro²  Tada, Michihiko¹  MacLennan, David H²  Asahi, Michio²  Kurzydlowski, Kazimierz² 

[1] Department of Medicine and Pathophysiology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;Banting and Best Department of Medical Research, University of Toronto, Charles H. Best Institute, Toronto, Ont. M5G 1L6, Canada

关键词: Ca2+-ATPase;    Phospholamban;    Cytochrome b 5;    Sarcoplasmic reticulum;   

DOI  :  10.1016/S0014-5793(98)00151-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A series of chimeras between the transmembrane domains of phospholamban (PLN) and cytochrome b ₅ were coexpressed with the Ca²⁺-ATPase of cardiac sarcoplasmic reticulum (SERCA2a). The chimeric molecules were not inhibitory, in line with our view that inhibitory PLN/SERCA2a interactions occur in transmembrane sequences, while cytoplasmic interactions regulate the inhibitory interactions in a four-base circuit.

【 授权许可】

Unknown