Co‐expression of slow‐twitch/cardiac muscle Ca2+‐ATPase (SERCA2) and phospholamban

FEBS Letters

Fujii, Junichi²  Tada, Michihiko¹  MacLennan, David H.²  Maruyama, Kei² 

[1] The First Department of Medicine and Pathophysiology, Osaka University School of Medicine, Fukushima-ku, Osaka 553, Japan;Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, 112 College Street, Toronto, Ontario M5G IL6, Canada

关键词: Ca2+-ATPase;    Phospholamban;    Co-expression;    Ca2+-affinity;   

DOI  :  10.1016/0014-5793(90)81092-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Full length cDNAs encoding both slow-twitch/cardiac (SERCA2) and fast-twitch skeletal muscle (SERCA1) Ca²⁺-ATPases were expressed by transient transfection of COS-1 cells. Studies of the Ca²⁺-dependency of Ca²⁺-transport in microsomes isolated from these cells showed that both isoforms had an affinity for Ca²⁺ of about 0.2 μM. The Ca²⁺-affinity of SERCA2 was lowered when phospholamban was co-expressed with it, demonstrating that the two proteins interact in this expression system. These studies support the view that phospholamban inhibition accounts for the low Ca²⁺-amnity and low activity of SERCA2 in cardiac muscle sarcoplasmic reticulum.

【 授权许可】

Unknown