Interaction of peroxynitrite with carotenoids and tocopherols within low density lipoprotein

FEBS Letters

Rice-Evans, Catherine²  Sampson, Julia¹  Pannala, Ananth Sekher¹  Singh, Surinder¹ 

[1] Department of Pharmacy, King's College, University of London, Manresa Road, Chelsea, London SW3 6LX, UK;International Antioxidant Research Centre, UMDS-Guy's Hospital, St. Thomas' Street, London SE1 9RT, UK

关键词: Carotenoid;    Low density lipoprotein oxidation;    Peroxynitrite;    Tocopherol;    Lipid peroxidation;    LDL;    low density lipoprotein;    α-TOH;    α-tocopherol;    α-TQ;    α-tocopheroquinone;    γ-TOH;    γ-tocopherol;    BHT;    butylated hydroxy toluene;    TBARS;    thiobarbituric acid reactive substances;    CV%;    percentage coefficient of variation;    M%D;    mean percentage difference;    MDA;    malondialdehyde;   

DOI  :  10.1016/S0014-5793(98)00108-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxynitrite is a powerful oxidising and nitrating agent generated in vivo by the combination of nitric oxide and superoxide. Previous studies have shown that on exposure to peroxynitrite, low density lipoprotein (LDL) is modified resulting in both a time- and concentration-dependent change to lipid and protein components. The present investigation highlights the reaction between carotenoids and tocopherols, present within the lipophilic phase of LDL, and peroxynitrite at varying concentrations. It was observed that the carotenoids were consumed by a significantly greater proportion than that of the tocopherols with lycopene (87.2±11%) being more reactive than β-carotene (68.2±5.8%) when exposed to peroxynitrite (50 μM) for 1 min. Among the tocopherols, α-tocopherol (54.9±20.2%) was more extensively depleted than γ-tocopherol (14.7±1.09%) at peroxynitrite concentration of 500 μM. It was also observed that peroxynitrite, unlike copper ions, does not lead to significant peroxidation of LDL as determined by the formation of conjugated dienes and thiobarbituric acid-reactive substances.

【 授权许可】

Unknown