Prostaglandin E2 biphasic control of lymphocyte proliferation: inhibition by picomolar concentrations

FEBS Letters

Gonchar, Maria V¹  Varfolomeyev, Sergey D¹  Sergeeva, Marina G¹  Mevkh, Alevtina T¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia

关键词: Prostaglandin E2;    Adaptation;    Macrophage;    Splenocyte;    Proliferation;   

DOI  :  10.1016/S0014-5793(97)01388-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prostaglandins (PGs) have an important physiological role in the modulation of various cell immune functions. The main sources of PGs during immune responses are monocyte cells. We report here the ability of non-stimulated macrophages to synthesize prostanoids and show that peritoneal mouse macrophages synthesize PGE₂, PGF_2a and thromboxane B₂, spleen macrophages produce PGE₂ and PGF_2a, and in a fresh medium this synthesis reaches a constant basal level in a few hours. We studied the kinetics of Con A-induced proliferation of murine splenocytes under the influence of a wide range of PGE₂ concentrations (10⁻¹⁴–10⁻⁷ M). The suppressive effect of PGE₂ decreased when its concentration was lowered and disappeared at 10⁻⁹ M PGE₂ (this concentration corresponded to the basal level of non-stimulated macrophage synthesis of PGE₂). Further lowering of the concentration became essential for the proliferation process once again, and at picomolar concentrations PGE₂ caused a suppressive effect comparable with that for 10⁻⁸ M PGE₂. We also found that PGE₂ significantly inhibited cell proliferation when it was added 1 h before the addition of mitogen, as compared with simultaneous mitogen addition. The effect was obtained for both low (10⁻¹² M) and high (10⁻⁸ M) PGE₂ concentrations. This phenomenon of PGE₂ biphasic control of lymphocyte proliferation may play an important role in cellular homeostasis, in particular in immune cell function regulation.

【 授权许可】

Unknown