Nuclear factor‐κB activation in human monocytes stimulated with lipopolysaccharide is inhibited by fibroblast conditioned medium and exogenous PGE2

FEBS Letters

Messina, Angelo¹  Bonaiuto, Corrada¹  Crimi, Nunzio²  Conte, Enrico¹  Nesci, Caterina¹  Vancheri, Carlo² 

[1] Institute of General Pathology, University of Catania, via Androne 83, 95125 Catania, Italy;Institute of Respiratory Disease, University of Catania, via Passo Gravina 187, 95125 Catania, Italy

关键词: Nuclear factor κB;    Monocyte;    Macrophage;    Lipopolysaccharide;    Fibroblast;    Prostaglandin E2;   

DOI  :  10.1016/S0014-5793(96)01409-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nuclear factor κB (NF-κB) is thought to be crucially involved in the gene activation of several cytokines, including tumor necrosis factor α (TNF). Previously, we showed that fibroblast conditioned medium (FCM) is able to inhibit both TNF mRNA accumulation and protein release in peripheral blood-derived human monocytes (PBM) stimulated with lipopolysaccharide (LPS). In this study we have investigated the effect of FCM on the LPS-induced DNA-binding activity of NF-κB, by means of electrophoretic shift assay (EMSA). We provide evidence that FCM strongly inhibits the LPS-induced NFκB activation in PBM. Furthermore, we show that exogenous PGE₂ mimics the NFκB inhibitory effect of FCM. On the other hand, FCM produced in the presence of indomethacin does not inhibit NF-κB activation by LPS. Our results lend further support to the hypothesis that inflammatory and immune responses of monocytes/macrophages may be modulated at the molecular level by signals originating from tissue structural cells such as fibroblasts.

【 授权许可】

Unknown