Activity of monomeric, dimeric, and trimeric flavonoids on NO production, TNF‐α secretion, and NF‐κB‐dependent gene expression in RAW 264.7 macrophages

FEBS Letters

Packer, Lester¹  Park, Young Chul¹  Saliou, Claude¹  Valacchi, Giuseppe¹  Rimbach, Gerald¹ 

[1] Department of Molecular and Cell Biology, 251 Life Sciences Addition, University of California, Berkeley, CA 94720-3200, USA

关键词: Tumor necrosis factor-α;    Nitric oxide;    Nuclear factor κB;    Macrophage;    Procyanidin;    IFN-γ;    interferon-γ;    iNOS;    inducible nitric oxide synthase;    LPS;    lipopolysaccharide;    NF-κB;    nuclear factor κB;    PCA;    procyanidin;    PYC;    Pycnogenol;    TNF-α;    tumor necrosis factor-alpha;   

DOI  :  10.1016/S0014-5793(99)01735-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flavonoids are potent antioxidants and have been associated with lowering the risk of cardiovascular diseases. In this study, the effect of flavonoids (monomers, dimers and a trimer) as well as French maritime pine bark extract, Pycnogenol, on NO production, tumor necrosis factor-α (TNF-α) secretion and nuclear factor (NF)-κB activity was compared. Monomers and dimers repressed NO production, TNF-α secretion and NF-κB-dependent gene expression induced by interferon γ, whereas the trimeric procyanidin C2 and Pycnogenol enhanced these parameters. In addition, in unstimulated RAW 264.7 macrophages, both procyanidin C2 and Pycnogenol increased TNF-α secretion in a concentration- and time-dependent manner. These results demonstrate that procyanidins act as modulators of the immune response in macrophages.

【 授权许可】

Unknown