hUBC9 associates with MEKK1 and type I TNF‐α receptor and stimulates NFκB activity

FEBS Letters

Munro, Robin¹  D'Alonzo, Jill¹  Marcireau, Christophe²  Stone, Maureen¹  Ressner, Rose¹  Saltzman, Alan¹  Jaye, Michael¹  Tocque, Bruno²  Franks, Carol¹  Ivashchenko, Yuri¹  Searfoss, George¹ 

[1] Rhône-Poulenc Rorer Central Research, Gene Medicine Department, 500 Arcola Rd., Collegeville, PA 19426, USA;Rhône-Poulenc Rorer, Vitry, France

关键词: Tumor necrosis factor-α;    Ubiquitin conjugating enzyme;    Yeast two-hybrid;    Nuclear factor κB;    MEKK1;   

DOI  :  10.1016/S0014-5793(98)00287-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two-hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF-α receptor, respectively. Because both of these proteins regulate NFκB activity, the role of hUBC9 in modulating NFκB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFκB as determined by NFκB reporter and IL-6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFκB reporter activity. Thus, hUBC9 modulates NFκB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF-α receptor.

【 授权许可】

Unknown