Journal of Inflammation | |
Δ9-Tetrahydrocannabinol (THC) enhances lipopolysaccharide-stimulated tissue factor in human monocytes and monocyte-derived microvesicles | |
Short Report | |
Thomas W. Klein1  Maureen M. Goodenow2  Bruce A. Goldberger2  Julie C. Williams2  John W. Sleasman3  Nigel Mackman4  | |
[1] Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, USA;Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, 2033 Mowry Road, 32610-3663, Gainesville, FL, USA;Department of Pediatrics, Division of Allergy, Immunology and Rheumatology, School of Medicine, Duke University, Durham, NC, USA;Division of Hematology and Oncology, Department of Medicine, McAlister Heart Institute, University of North Carolina, Chapel Hill, NC, USA; | |
关键词: Lipopolysaccharide; Marijuana; Monocyte; Tissue factor; THC; | |
DOI : 10.1186/s12950-015-0084-1 | |
received in 2015-03-05, accepted in 2015-06-04, 发布年份 2015 | |
来源: Springer | |
【 摘 要 】
BackgroundImmunomodulatory effects in humans of Δ9−Tetrahydrocannabinol (THC), the psychoactive component of marijuana are controversial. Tissue factor (TF), the activator of the extrinsic coagulation cascade, is increased on circulating activated monocytes and is expressed on microvesicles released from activated monocytes during inflammatory conditions, which perpetuate coagulopathies in a number of diseases. In view of the increased medicinal use of marijuana, effects of THC on human monocytes and monocyte-derived microvesicles activated by lipopolysaccharide (LPS) were investigated.FindingsPeak levels of TF procoagulant activity developed in monocytes or microvesicles 6 h following LPS treatment and were unaltered by THC. After 24 h of LPS stimulation, TF activity declined in control-treated or untreated cells and microvesicles, but persisted with THC treatment. Peak TF protein occurred within 6 h of LPS treatment independent of THC; by 24 h, TF protein declined to almost undetectable levels without THC, but was about 4-fold greater with THC. Steady-state TF mRNA levels were similar up to 2 h in the presence of LPS with or without THC, while 10-fold greater TF mRNA levels persisted over 3–24 h with THC treatment. Activation of MAPK or NF-κB pathways was unaltered by THC treatment and inflammatory cytokine IL-6 levels were unchanged. In contrast, TNF and IL-8 levels were enhanced by 20–50 %.ConclusionsTHC enhances TF expression in activated monocytes resulting in elevated procoagulant activity. Marijuana use could potentiate coagulopathies in individuals with chronic immune activation such as HIV-1 infection or inflammatory bowel disease.
【 授权许可】
Unknown
© Williams et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202311102332604ZK.pdf | 813KB | download |
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]