12‐Hydroxyeicosatetraenoic acid upregulates P‐selectin‐induced tissue factor activity on monocytes

FEBS Letters

Pellegrini, Giuliana³  Malandra, Riccardo³  Furie, Barbara C.²  Celi, Alessandro¹  Lorenzet, Roberto³  Furie, Bruce² 

[1] Dipartimento di Cardiologia, Angiologia e Pneumologia, U.O. Fisiopatologia Respiratoria, Università di Pisa, Pisa, Italy;Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02111, USA;‘Antonio Taticchi' Unit for Atherosclerosis and Thrombosis, Department of Vascular Medicine and Pharmacology, Istituto Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, Via Nazionale, 66030 S. Maria Imbaro, Italy

关键词: Tissue factor;    P-selectin;    12-Hydroxyeicosatetraenoic acid;    Monocyte;    12-HETE;    12-hydroxyeicosatetraenoic acid;    5-HETE;    5-(S)-hydroxyeicosatetraenoic acid;    LTC4;    leukotriene C4;   

DOI  :  10.1016/S0014-5793(98)01610-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

12-Hydroxyeicosatetraenoic acid (12-HETE), a product of the platelet lipoxygenase pathway, amplifies tissue factor expression by P-selectin-stimulated monocytes in a time- and dose-dependent fashion. The same effect is observed when monocytes are incubated with Chinese hamster ovary cells transfected with the P-selectin cDNA. Both 5-HETE and leukotriene C₄ are inactive in this system. Furthermore, the effect is not dependent on non-specific monocyte adhesion, since monocytes incubated with CHO cells expressing E-selectin do not express tissue factor, either in the presence or in the absence of 12-HETE. These results show that 12-HETE is a cofactor for the expression of tissue factor by monocytes.

【 授权许可】

Unknown