Modulation of fructose‐2,6‐bisphosphate metabolism by components of the extracellular matrix in cultured cells. Interaction with epidermal growth factor

FEBS Letters

Baulida, Josep¹  Onetti, Rafael¹  Bassols, Anna¹ 

[1] Departament de Bioquı́mica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain

关键词: Fructose-2;    6-bisphosphate;    Epidermal growth factor;    Laminin;    Heparin;    ECM;    extracellular matrix;    Fru-2;    6-P2;    fructose-2;    6-bisphosphate;    PFK-1;    6-phosphofructo-1-kinase;    PFK-2;    6-phosphofructo-2-kinase;    EGF;    epidermal growth factor;    PLC;    phospholipase C;    PK-C;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol 13-acetate;    TRE;    TPA-responsive element;   

DOI  :  10.1016/S0014-5793(97)01338-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The use of NIH3T3 fibroblasts overexpressing different mutations of the EGF receptor shows that regulation of fructose-2,6-bisphosphate (Fru-2,6-P₂) metabolism by EGF is mediated by the kinase activity of the EGF receptor and suggests a PLCγ1-mediated mechanism. The effect of several extracellular matrix components on glucose metabolism was assessed by incubating A431 cells and NIH3T3 fibroblasts with heparin, laminin, fibronectin, collagen and PG-I and PG-II proteoglycans and measuring the levels of Fru-2,6-P₂. Laminin increased the levels of Fru-2,6-P₂ and heparin decreased the levels of the metabolite, whereas the other molecules did not have any effect. No effect of laminin or heparin in glucose uptake by the cell was observed. Laminin was able to modulate the effects of EGF on Fru-2,6-P₂ concentration, suggesting cross-talk between these agents.

【 授权许可】

Unknown