Inhibition of MAP kinase blocks insulin‐mediated DNA synthesis and transcriptional activation of c‐fos by Elk‐1 in vascular smooth muscle cells

FEBS Letters

Xi, Xiao-Ping¹  Goetze, Stephan²  Law, Ronald E¹  Graf, Kristof²  Hsueh, Willa A¹ 

[1] University of California, Los Angeles, School of Medicine, Division of Endocrinology, Diabetes and Hypertension, Warren Hall, 2nd Floor, Room 24-122, 900 Veteran Avenue, MC 178622, Los Angeles, CA 90024, USA;Department of Medicine/Cardiology, Virchow Klinikum HU Berlin and German Heart Institute, Berlin, Germany

关键词: Vascular smooth muscle cell;    Insulin;    c-fos;    Serum response element;    Elk-1;    Mitogen-activated protein kinase;   

DOI  :  10.1016/S0014-5793(97)01303-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin-stimulated DNA synthesis, MAP kinase (MAPK) activity and c-fos expression in vascular smooth muscle cells (VSMCs) was blocked by the MAPK inhibitor PD 98059. Regulation of c-fos expression by the transcription factor Elk-1 at the serum response element (SRE) is dependent on its phosphorylation by MAPK. PD 98059 also suppressed insulin-induced Elk-1 transcriptional activity through the SRE. These data show that MAPK plays a critical role in both insulin-mediated growth and Elk-1-dependent induction of c-fos in VSMCs.

【 授权许可】

Unknown