Essential role of Rac GTPase in hydrogen peroxide‐induced activation of c‐fos serum response element

FEBS Letters

Kim, Byung-Chul²  Kang, Il-Jun¹  Choi, Sung-E²  Kim, Jae-Hong²  Kwack, Hee-Jin²  Kumar, Chandra C.³  Kim, Yong-Sun² 

[1] Department of Nutrition, Hallym University, Chun-Cheon, Kangwon-do 200-702, South Korea;Laboratory of Molecular and Cellular Genetics, Institute of Environment and Life Science, Hallym University, Chun-Cheon, Kangwon-do 200-702, South Korea;Tumor Biology Department, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA

关键词: Hydrogen peroxide;    Rac GTPase;    c-fos;    Serum response element;    Phospholipase A2;    Arachidonic acid;   

DOI  :  10.1016/S0014-5793(97)00249-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H₂O₂ stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A₂ (PLA₂) in the H₂O₂-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA₂, blocked H₂O₂-induced SRE activation dramatically. Together, these findings suggest a critical role of ‘Rac and subsequent activation of phospholipase A₂’ in the signaling pathway of H₂O₂ to SRE.

【 授权许可】

Unknown