Role of Rac GTPase in the nuclear signaling by EGF

FEBS Letters

Kim, Byung-Chul¹  Kim, Jae-Hong¹ 

[1] Laboratory of Molecular and Cellular Genetics, Institute of Environment and Life Science, Hallym University, Chun-Cheon, Kangwon-do 200-702, South Korea

关键词: Signal transduction;    Epidermal growth factor;    Rac GTPase;    c-fos;    Serum response element;    Phospholipase A2;   

DOI  :  10.1016/S0014-5793(97)00289-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of Rac in epidermal growth factor (EGF)-induced c-fos serum response element (SRE) activation was examined in Rat-2 fibroblast cells. By reporter gene analysis following transient or stable transfections with pEXV-RacN17 encoding a dominant-negative mutant of Rac, EGF-induced activation of c-fos SRE-luciferase gene was shown to be selectively inhibited, suggesting that Rac activity is necessary for the full activation of SRE by EGF. Our further study to analyze the downstream mediator of Rac in EGF-signaling cascade demonstrated that there is a functional link between Rac and phospholipase A₂ (PLA₂) activation and further that PLA₂ mediates, at least partly, the Rac signaling to SRE. Together, our results point to a critical role of Rac and Rac-activated PLA₂ in the EGF-signaling cascade to c-fos SRE. We propose that `Rac-PLA₂' cascade is one of the major signaling pathways by which EGF stimulates c-fos SRE.

【 授权许可】

Unknown