Activation of Raf‐1 is defective in annexin 6 overexpressing Chinese hamster ovary cells

FEBS Letters

Kirchhoff, Matthias¹  Grewal, Thomas¹  Tebar, Francesc²  Enrich, Carlos²  Peiró, Sandra²  Pons, Mònica²  de Diego, Iñaki² 

[1] Medizinische Kernklinik und Poliklinik, Universität-Krankenhaus Eppendorf, University of Hamburg, Hamburg, Germany;Departament de Biologia Cellular, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036 Barcelona, Spain

关键词: Annexin 6;    Raf-1;    Epidermal growth factor;    Ras;    Signal transduction;    12-O-Tetradecanoyl-phorbol-13-acetate;    C2;    conserved region 2;    GAP;    GTPase activating protein;    TPA;    12-O-tetradecanoyl-phorbol-13-acetate;    GST;    glutathione-S-transferase;    CHO;    Chinese hamster ovary cells;    RBD;    Ras-binding domain;    MBP;    myelin basic protein;    PS;    phosphatidylserine;   

DOI  :  10.1016/S0014-5793(01)02635-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Annexin 6 is a Ca²⁺-dependent phospholipid-binding protein involved in membrane trafficking. In this study we demonstrate the association of Raf-1 with recombinant rat annexin 6. Raf–annexin 6 interaction was shown to be independent of cell activation by epidermal growth factor (EGF) or phorbol esters (12-O-tetradecanoyl-phorbol-13-acetate (TPA)). A stable Chinese hamster ovary (CHO)-anx6 cell line overexpressing annexin 6 was established to examine the function of annexin 6. In these cells, no increase of Ras-GTP levels, induced by EGF or TPA, was detected. In addition, the activity of Raf was completely inhibited, whereas the mitogen-activated protein kinase-P was unaffected.

【 授权许可】

Unknown