Opposite effects of cell differentiation and apoptosis on Ap3A/Ap4A ratio in human cell cultures

FEBS Letters

Kisselev, Lev¹  Prudovsky, Igor¹  Suzuki, Hisanori²  Dal Pra, Ilaria²  Vartanian, Amalia¹ 

[1] Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, 117984 Moscow, Russia;Facolta di Medicina e Chirurgia, Universita di Verona, Verona, Italy

关键词: Human cell H60;    Apoptosis;    Macrophage differentiation;    Interferon;    Diadenosine oligophosphate;    Phorbol ester;    Signal transduction;    Ap3A;    P1;    P3-bis(5′-adenosyl)triphosphate;    Ap4A;    P1;    P4-bis(5′-adenosyl)tetraphosphate;    ARS;    aminoacyl-tRNA synthetases (E.C. 6.1.1);    DAOP;    diadenosine oligophosphates;    PBS;    150 mM NaCl in 150 mM sodium phosphate buffer;    pH 7.2;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    VP16;    Epipodophyllotoxin;   

DOI  :  10.1016/S0014-5793(97)01086-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biological role of diadenosine oligophosphates (DAOP) remains obscure in spite of numerous attempts to solve this enigma. It is known that Ap₃A contrary to Ap₄A accumulates in human cultured cells treated with interferons (IFNs) alpha or gamma. Since IFNs are considered as antiproliferative regulators, we assumed that different cell status may be associated with varying intracellular levels of DAOP. Promyelocytic human cell line HL60 induced by phorbol ester (TPA) to differentiate to macrophage-like cells in culture exhibits a profound loss of proliferative potential. Here we have shown a 4–5-fold increase in Ap₃A concentration in HL60 cells induced by TPA, similar to the effect of IFN, while the Ap₄A concentration remained unchanged. On the contrary, in cells undergoing apoptosis induced by VP16, a topoisomerase II inhibitor, the Ap₃A concentration considerably decreased, while the Ap₄A concentration increased. These findings combined with earlier results suggest an involvement of the Ap₃A/Ap₄A ratio in signal transduction pathways controlling the cell status.

【 授权许可】

Unknown