期刊论文详细信息
FEBS Letters
Chloroplastic prenylated proteins
Swiezewska, Ewa1  Shipton, Catherine Ann2  Parmryd, Ingela2  Andersson, Bertil2  Dallner, Gustav2 
[1] Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland;Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden
关键词: Chloroplast;    Farnesyl protein transferase;    Prenylation;   
DOI  :  10.1016/S0014-5793(97)01063-6
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

By in vivo [3H]mevalonate labelling of spinach combined with biochemical analysis, evidence is provided for the existence of protein prenylation in chloroplasts. Approximately 20 prenylated polypeptides were resolved by SDS-PAGE followed by autoradiography. Thermolysin treatment of intact chloroplasts revealed that about 40% of the prenylated polypeptides were associated with the cytoplasmic surface of the outer envelope membrane. The remaining portion was present in thylakoids and/or the inner envelope membrane. The majority of the prenylated polypeptides were associated with larger membrane protein complexes. A farnesyl protein transferase activity was found to be associated with the thylakoid membrane.

【 授权许可】

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