Chloroplastic prenylated proteins

FEBS Letters

Swiezewska, Ewa¹  Shipton, Catherine Ann²  Parmryd, Ingela²  Andersson, Bertil²  Dallner, Gustav² 

[1] Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland;Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Chloroplast;    Farnesyl protein transferase;    Prenylation;   

DOI  :  10.1016/S0014-5793(97)01063-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By in vivo [³H]mevalonate labelling of spinach combined with biochemical analysis, evidence is provided for the existence of protein prenylation in chloroplasts. Approximately 20 prenylated polypeptides were resolved by SDS-PAGE followed by autoradiography. Thermolysin treatment of intact chloroplasts revealed that about 40% of the prenylated polypeptides were associated with the cytoplasmic surface of the outer envelope membrane. The remaining portion was present in thylakoids and/or the inner envelope membrane. The majority of the prenylated polypeptides were associated with larger membrane protein complexes. A farnesyl protein transferase activity was found to be associated with the thylakoid membrane.

【 授权许可】

Unknown